Abstract:
Stevioside is a non-cariogenic and low-calorigenic diterpenoid glycoside has slightly bitterness and bad after taste.
Enzymatic modification by alternansucrase from Leuconostoc citreum SK24.002 was utilized in biotransformation
of stevioside to remove the bitter taste fully or partially and an aftertaste of the stevioside, using sucrose as donor
and stevioside as acceptor. Tri-glucosyl-stevioside produced during alternansucrase acceptor reaction of
stevioside were successfully separated using adsorption separation technology of macroporous adsorption resin
(AB-8) flowed by semipreparative high performance liquid chromatography. Structure of the product was
characterized to be 13-{[α-D-glucopyranosyl-(1→3)-α-D-glucopyranosyl-(1→6)-α-D-glucopyranosyl-(1→3)-β-Dglucopyranosyl-(
1→2)-β-D-glucopyranosyl]oxy}kaur-16-en-19-oic acid β-D glucopyranosyl ester on the basis of
extensive one dimension and two dimensional NMR and LC-ESI-MS in negative mode data.
