Phenotypic detection of Extended Spectrum βeta-Lactamase producing Escherichia coli isolated from urinary tract infected Patients in Khartoum, Sudan

Abstract

The aim of this study was to determine the frequency of extended-spectrum β-lactamases (ESBLs) in uropathogenic E. coli isolates. A total of 100 E. coli isolates were obtained, among which 43(43%) and 57(57%) were collected from male and female, respectively. Also the distribution of these samples among age group were children 14 (14%), adult 41 (41%) and geriatric 45(45%). All isolates were screened and confirmed for the presence of ESBL phenotypically with Double Disk Synergy Test, and tested for their susceptibility to non Beta-lactam antibiotics. Among the 100 E. coli isolates, only 35% were judged as ESBLs-positive strains. The frequency of ESBLs production was not significantly different among gender as their frequency were 30%(13/43) in male and 38%(22/57) in female; and among age as their frequency were 29%(4/14) in children, 34%(14/41) in adult and 37% (17/45) in geriatric. ESBLS producer E. coli were significantly more resistant to Ciprofloxacin, Nitrofurontoin, Gentamycin and Tetracycline. Also Most ESBLs producing E. coli isolates were still susceptible to Impenem, Nitrofurontion Gentamycin and Amikacin. Our results suggest that the frequency of ESBLs among uropathogenic E. coli is currently in progress in Sudan, and there for further studies is needed.